? <div>Exploration of the key microbes and composition stability of microbial consortium GF-20 with efficiently decomposes corn stover at low temperatures</div> 亚博yabo娱乐平台,yabo sport,yabo2018体育
JIA
??????
Quick Search in JIA? ?? ?Advanced Search??
? ? 2019, Vol. 18 Issue (8): 1893-1904???? DOI: 10.1016/S2095-3119(19)62609-2
Crop Science Current Issue | Next Issue | Archive | Adv Search ?|? ?
Exploration of the key microbes and composition stability of microbial consortium GF-20 with efficiently decomposes corn stover at low temperatures
YU Xiao-fang*, BORJIGIN Qinggeer*, GAO Ju-lin, WANG Zhi-gang, HU Shu-ping, BORJIGIN Naoganchaolu, WANG Zhen, SUN Ji-ying, HAN Sheng-cai
Agricultural College, Inner Mongolia Agricultural University, Hohhot 010019, P.R.China
?Download: PDF in ScienceDirect (0 KB) ? HTML (1 KB) ? Export: BibTeX | EndNote (RIS) ? ???Supporting Info
Abstract?
The microbial consortium GF-20 (GF-20) can efficiently decompose corn stover at low temperatures.? The present study explored the key microbes of GF-20 and evaluated different culture conditions on its composition stability to promote the utilization of corn stover decomposing microbes in low temperature regions.? GF-20 was subcultured to the 15th generation under different temperatures, pHs, carbon, and nitrogen sources.? Then, the dynamics of fermenting pH, cellulose enzyme activities, carbohydrate concentration, and oxidation reduction potential were determined to estimate the degradation efficiency of corn stover with GF-20.? Furthermore, the structural stability and functional microbes of GF-20 were identified on the basis of PCR-denaturing gradient gel electrophoresis (DGGE) profiling and principal component analysis.? The results showed that the offspring of GF-20 subcultured under different temperatures (4–30°C) and pH (6.0–9.0) conditions maintained stable growth, decomposition function, and composition structure.? Furthermore, consortia GF-20 had a stable composition structure, which induced GF-20 to secrete cellulose and promote substrate decomposition as corn stover and ammonium were used as sources of carbon and nitrogen, respectively.? According to the PCR-DGGE profiles, the key strains of GF-20 were determined to be Bacillus licheniformis, Cellvibrio mixtus subsp. mixtus, Bacillus tequilensis, Clostridium populeti, and Clostridium xylanolyticum.
?
Service
E-mail this article
Add to my bookshelf
Add to citation manager
E-mail Alert
RSS
Articles by authors
YU Xiao-fang
BORJIGIN Qinggeer
GAO Ju-lin
WANG Zhi-gang
HU Shu-ping
BORJIGIN Naoganchaolu
WANG Zhen
SUN Ji-ying
HAN Sheng-cai
Key wordsmicrobial consortium ?? ?decomposition of corn stalks??? ?low temperature??? ?functional microbe??? ?structure stability?? ??
Received: 2018-10-25; Accepted: 2018-12-10
Fund: This study was supported by the National Natural Science Foundation of China (31760353 and 31560360), the National Key R&D Program of China (2017YFD0300804 and 2016YFD0300103), the earmarked fund for China Agriculture Research System (CARS-02-63), the Crop Science Observation & Experiment Station in Loess Plateau of North China, Ministry of Agriculture, China (25204120), and the Advanced Talented Scholars of Inner Mongolia Agricultural University, China (NDYB2016-15).
Corresponding Authors: Correspondence GAO Ju-lin, Tel: +86-471-4301317, E-mail: nmgaojulin@163.com ???
About author: * These authors contributed equally to this study.
Cite this article: ??
YU Xiao-fang, BORJIGIN Qinggeer, GAO Ju-lin, WANG Zhi-gang, HU Shu-ping, BORJIGIN Naoganchaolu, WANG Zhen, SUN Ji-ying, HAN Sheng-cai. 2019.
Exploration of the key microbes and composition stability of microbial consortium GF-20 with efficiently decomposes corn stover at low temperatures
. Journal of Integrative Agriculture, 18(8): 1893-1904.
URL: ?
http://www.chinaagrisci.com/Jwk_zgnykxen/EN/10.1016/S2095-3119(19)62609-2 ?????or ????http://www.chinaagrisci.com/Jwk_zgnykxen/EN/Y2019/V18/I8/1893
 
No references of article
[1] JIAO Xiao-qiang, WANG Chong, ZHANG Fu-suo. Editorial – Science and Technology Backyard: A novel model for technology innovation and agriculture transformation towards sustainable intensification[J]. Journal of Integrative Agriculture, 2019, 18(8): 1655-1656.
Copyright ? 2015 ChinaAgriSci.com, All Rights Reserved
Chinese Academy of Agricultural Sciences (CAAS) No. 12 South Street, Zhongguancun, Beijing 100081, P. R. China
http://www.ChinaAgriSci.com ??JIA E-mail: jia_journal@caas.cn