? <div>A rapid, simple, and sensitive immunoagglutination assay with silica nanoparticles for serotype identification of <em>Pseudomonas aeruginosa</em></div> 亚博yabo娱乐平台,yabo sport,yabo2018体育
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? ? 2019, Vol. 18 Issue (8): 1936-1943???? DOI: 10.1016/S2095-3119(18)62135-5
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A rapid, simple, and sensitive immunoagglutination assay with silica nanoparticles for serotype identification of Pseudomonas aeruginosa
DONG Xiu-mei1, 2*, TAO Jing1*, LI Ting-ting1, ZHANG Ping1, 2, ZHU Yan1, 2, TANG Yu1, SU Rui-hong1, SHI Dong-fang1, 2?
1 College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, P.R.China
2 Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, P.R.China
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Abstract?
An agglutination test based on colored silica nanoparticles (colored SiNps) was established to detect serotypes of Pseudomonas aeruginosa.? Monodisperse colored SiNps were used as agglutination test carriers.? The colored SiNps were prepared through reverse microemulsion with reactive dyes, sensitized with 11 kinds of mono-specific antibodies against P.?aeruginosa, and denoted as IgG-colored SiNps.? Eleven kinds of IgG-colored SiNps were individually mixed with P.?aeruginosa on a glass slide.? Different serotypes of P. aeruginosa could be identified by agglutination test with evident agglutination.? The P. aeruginosa could be detected in a range from 3.6×105 to 3.6×1012 cfu mL–1.? This new agglutination test was confirmed to be a speci?c, sensitive, fast, easy-to-perform, and cost-ef?cient tool for the routine diagnosis of P.?aeruginosa.
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DONG Xiu-mei
TAO Jing
LI Ting-ting
ZHANG Ping
ZHU Yan
TANG Yu
SU Rui-hong
SHI Dong-fang
Key words?Pseudomonas aeruginosa ?? ?colored silica nanoparticles??? ?agglutination test??? ?serotype detection?? ??
Received: 2018-08-21; Accepted: 2018-11-07
Fund:
This research was supported by the Open Project of the State Key Laboratory of Veterinary Etiological Biology, China (SKLVEB 2013 KFKT 0015).
Corresponding Authors: Correspondence SHI Dong-fang, Tel: +86-451-55190125, Fax: +86-451-55191200, E-mail: shidf@neau.edu.cn???
About author: DONG Xiu-mei, Tel: +86-451-55190125, E-mail: neaudxm@163.com; TAO Jing, Tel: +86-451-55190125, E-mail: taotao19920403 @126.com; * These authors contributed equally to this study.
Cite this article: ??
DONG Xiu-mei, TAO Jing, LI Ting-ting, ZHANG Ping, ZHU Yan, TANG Yu, SU Rui-hong, SHI Dong-fang . 2019.
A rapid, simple, and sensitive immunoagglutination assay with silica nanoparticles for serotype identification of Pseudomonas aeruginosa
. Journal of Integrative Agriculture, 18(8): 1936-1943.
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http://www.chinaagrisci.com/Jwk_zgnykxen/EN/10.1016/S2095-3119(18)62135-5 ?????or ????http://www.chinaagrisci.com/Jwk_zgnykxen/EN/Y2019/V18/I8/1936
 
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[1] JIAO Xiao-qiang, WANG Chong, ZHANG Fu-suo. Editorial – Science and Technology Backyard: A novel model for technology innovation and agriculture transformation towards sustainable intensification[J]. Journal of Integrative Agriculture, 2019, 18(8): 1655-1656.
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